Coquerel’s giant mouse lemurs primarily have a “scramble” method of mating, in which their sex ratios are roughly equivalent (although one study found that they may be slightly biased in favor of females) and males and females are evenly distributed throughout the habitat. This is in contrast to the contest model, in which there are many females that tend to stay congregated, and males that compete for mating opportunities.

Females of the species may become pregnant at any time of the year, although peak mating season begins in November and lasts through the spring. During this period of increased mating activity, males quadruple their typical home ranges and have more injuries than females. Males use breeding calls to attract mates, and, in preparation for mating, their testicles grow significantly—up to five times their usual size. Females are fertile for one day every 19 to 30 days. Females often mate with more than one male and can even give birth to a single litter fathered by multiple males.

Coquerel’s giant mouse lemurs don’t form pair bonds, and after mating, the female takes on the role as the sole provider to her offspring. Their gestation period is three months, after which one or two infants are typically born. They leave their nest at about three weeks of age. Mothers nurse their young multiple times per day, and they have to return back to their nest several times every night while foraging. Young become sexually mature when they are about a year old. It is unknown how long offspring stay with their mothers. In one study, a female frequently shared a nest with a male, believed to be her son, until the male was over two years old. Their generation length is 5 years.